|
KMID : 0984920150170020121
|
|
Journal of Skin Barrier Research
2015 Volume.17 No. 2 p.121 ~ p.121
|
|
|
Increased Activity of TRPV3 in Keratinocytes in Burn Scars with Postburn Pruritus
|
|
Cho Yong-Se
Park Sook-Young
Yang Yoon-Seok
Chung Bo-Young
Park Chun-Wook
Kim Hye-One
|
|
|
Abstract
|
|
|
|
Postburn pruritus is a common distressing consequence of burn wounds. Empirical antipruritic treatment often fails to have a satisfactory outcome on postburn pruritus, as the mechanism of postburn pruritus has not been fully elucidated. Our previous study had shown that expression of TRPV3 and TRPA1 were markedly increased in burn scars with postburn pruritus than those without it. The aim of this study was to evaluate the manifestation of TRPV3 and TRPA1 in postburn pruritus. Total 51 patients were divided into 2 groups: those with pruritus and those without it. Skin samples were obtained from 2 different sites (burn scars and normal skin). Keratinoocytes (KCs) and fibroblast from those samples were separated. Immunohistochemical staining for TRPV3 and TRPA1 and immunofluorescence staining for loricrin and involucrin were performed in burn scars and normal skin. And qPCR and western blotting of TRPV3, TRPA1, PAR2, NK1R, loricrin, involucrin, TGF beta and alpha SMA were done.. We also measured intracellular Ca(2+) levels following TRPV3 activation and blocking in KCs from scars with or without pruritus. As a result, Anti-TPRV3 was stained stronger in the epidermis of the burn scars of postburn pruritus patients than in those of non-pruritic burn patients. The mRNA and protein levels of TRPV3, PAR2 and NK1R were significantly increased in KCs from pruritic burn scars than in KCs from nonpruritic scars. With TRPV3 activation, intracellular Ca(2+) concentrations were significantly increased only in KCs from pruritic burn scars. We confirmed that TRPV3 of KCs contrubutes to the pathophysiology of postburn pruritus. These results may help determine a novel mechanism for postburn pruritus.
|
|
|
KEYWORD
|
|
|
Intracellular Ca2+, Postburn pruritus, Scar, TRPV3
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|
|
|